Endocrine Abstracts

In human GL cells, 11beta-HSD catalyses the interconversion of cortisol (F) with its inert 11-ketosteroid metabolite, cortisone (E). Since GL cells synthesise prostaglandins (PGs), and PGs have been shown to modulate the expression and activity of the cloned 11beta-HSD enzymes, the objective of the present study was to establish whether locally synthesised PGs affected the net oxidation of F to E by 11beta-HSD in human GL cells recovered from the ovaries of women undergoing co...

PGE2 stimulates progesterone synthesis in the primate corpus luteum, apparently via cAMP. Human GL cells express functional EP1 and EP2 receptors via which PGE2 elevates the intracellular calcium and cAMP concentrations respectively (Harris et al, 2001, BBRC 285:1089). In the present study, we have determined the involvement of EP1 and EP2 receptors in the stimulation of cAMP accumulation and progesterone synthesis in these human ovarian cel...